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CD7 Polyclonal antibody

CD7 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 11320-1-AP

Print datasheet

Synonyms

T cell surface antigen Leu-9, T cell leukemia antigen, T cell antigen CD7, LEU-9, GP40

验证数据展示

  • IHC staining of human tonsillitis using 11320-1-AP

    IHC staining of human tonsillitis using 11320-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11320-1-AP (CD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD7 antibody included
  • IHC staining of human tonsillitis using 11320-1-AP

    IHC staining of human tonsillitis using 11320-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11320-1-AP (CD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD7 antibody included
  • IF Staining of human tonsillitis using 11320-1-AP

    IF Staining of human tonsillitis using 11320-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD7 antibody (11320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human tonsillitis using 11320-1-AP

    IF Staining of human tonsillitis using 11320-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD7 antibody (11320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD7 Ready-To-Use IHC Kit. CD7 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11320-1-AP targets CD7 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen CD7 fusion protein Ag0486 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD7 molecule
Synonyms T cell surface antigen Leu-9, T cell leukemia antigen, T cell antigen CD7, LEU-9, GP40
Calculated Molecular Weight 240 aa, 25 kDa
GenBank Accession NumberBC009293
Gene Symbol CD7
Gene ID (NCBI) 924
ENSEMBL Gene IDENSG00000173762
RRIDAB_2877758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09564
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD7 is a 40 kD type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.

实验方案

Product Specific Protocols
IHC protocol for CD7 antibody 11320-1-APDownload protocol
IF protocol for CD7 antibody 11320-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols