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验证数据展示

  • IHC staining of human appendicitis using 60209-1-Ig

    IHC staining of human appendicitis using 60209-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD7 antibody included
  • IHC staining of human appendicitis using 60209-1-Ig

    IHC staining of human appendicitis using 60209-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD7 antibody included
  • IF Staining of human tonsillitis using 60209-1-Ig

    IF Staining of human tonsillitis using 60209-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60209-1-Ig (CD7 antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 60209-1-Ig

    IF Staining of human tonsillitis using 60209-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60209-1-Ig (CD7 antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CD7 Monoclonal antibody

CD7 Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 60209-1-Ig

产品说明书

CloneNo. 1G10D8
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IHC, IF-P, ELISA and More (1)

别名

T cell leukemia antigen, T cell antigen CD7, LEU-9, GP40, CD7 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman appendicitis tissue, human lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD7 Ready-To-Use IHC Kit. CD7 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:1600-1:6400
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60209-1-Ig targets CD7 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, ELISA Application Description
文献引用应用IF
经测试反应性 human
免疫原 CD7 fusion protein Ag1805 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD7 molecule
别名 T cell leukemia antigen, T cell antigen CD7, LEU-9, GP40, CD7 molecule
计算分子量 240 aa, 25 kDa
GenBank蛋白编号BC009293
基因名称 CD7
Gene ID (NCBI) 924
ENSEMBL Gene IDENSG00000173762
RRIDAB_10953398
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09564
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD7 is a 40 kD type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.

实验方案

Product Specific Protocols
WB protocol for CD7 antibody 60209-1-IgDownload protocol
IHC protocol for CD7 antibody 60209-1-IgDownload protocol
IF protocol for CD7 antibody 60209-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF,FC

Cell Metab

Bone marrow immune cells respond to fluctuating nutritional stress to constrain weight regain

Authors - Hai-Yan Zhou
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Small

In Vivo mRNA CAR T Cell Engineering via Targeted Ionizable Lipid Nanoparticles with Extrahepatic Tropism

Authors - Margaret M Billingsley
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