CD99 Polyclonal antibody
CD99 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, U-937 cells, THP-1 cells |
Positive IHC detected in | human pancreas tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 3 publications below |
IF | See 1 publications below |
产品信息
The immunogen of 23079-1-AP is CD99 Fusion Protein expressed in E. coli.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | IF, IHC |
Tested Reactivity | human |
Cited Reactivity | human, pig |
Immunogen | CD99 fusion protein Ag19461 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CD99 molecule |
Synonyms | 12E7, CD99, CD99 antigen, CD99 molecule, E2 antigen, MIC2, MIC2X, MIC2Y, Protein MIC2, T cell surface glycoprotein E2 |
Calculated Molecular Weight | 19 kDa, 16 kDa, 17 kDa |
Observed Molecular Weight | 28 kDa, 32 kDa |
GenBank Accession Number | BC021620 |
Gene Symbol | CD99 |
Gene ID (NCBI) | 4267 |
RRID | AB_2879205 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen Affinity purified |
UNIPROT ID | P14209 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CD99 antibody 23079-1-AP | Download protocol |
IHC protocol for CD99 antibody 23079-1-AP | Download protocol |
FC protocol for CD99 antibody 23079-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Anim Sci Biotechnol Single-cell RNA-sequencing reveals the dynamic process and novel markers in porcine spermatogenesis. | ||
Oncol Lett Metastatic extraskeletal Ewing's sarcoma treated with trabectedin: A case report. | ||