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P-cadherin Monoclonal antibody

P-cadherin Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

1G10F7

Cat No : 66295-1-Ig

Print datasheet

Synonyms

Cadherin 3, CDH3, CDHP, HJMD, P cadherin, PCAD, P-cadherin, Placental cadherin

验证数据展示

  • WB analysis of A431 using 66295-1-Ig

    WB analysis of A431 using 66295-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 66295-1-Ig (P-cadherin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 66295-1-Ig

    IHC staining of human placenta using 66295-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66295-1-Ig (P-cadherin antibody) at dilution of 1:250 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 66295-1-Ig

    IHC staining of human placenta using 66295-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66295-1-Ig (P-cadherin antibody) at dilution of 1:250 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of A431 using 66295-1-Ig

    FC experiment of A431 using 66295-1-Ig

    1X10^6 A431 cells were stained with 0.20ug P-cadherin antibody (66295-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

经过测试的应用

Positive WB detected inA431 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66295-1-Ig targets P-cadherin in WB, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IHC, WB,ELISA
Tested Reactivity human
Immunogen P-cadherin fusion protein Ag4758 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cadherin 3, type 1, P-cadherin (placental)
Synonyms Cadherin 3, CDH3, CDHP, HJMD, P cadherin, PCAD, P-cadherin, Placental cadherin
Calculated Molecular Weight 829 aa, 91 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC041846
Gene Symbol CDH3
Gene ID (NCBI) 1001
RRIDAB_2881678
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22223
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.

实验方案

Product Specific Protocols
WB protocol for P-cadherin antibody 66295-1-IgDownload protocol
IHC protocol for P-cadherin antibody 66295-1-IgDownload protocol
FC protocol for P-cadherin antibody 66295-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols