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验证数据展示

  • WB analysis of Neuro-2a using 27058-1-AP

    WB analysis of Neuro-2a using 27058-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27058-1-AP (CDK5R2/p39 antibody at dilution of 1:600 incubated at 4 degree celsius over night.

  • WB analysis using 27058-1-AP

    WB analysis using 27058-1-AP

    various lysates were subjected to SDS PAGE followed by western blot with 27058-1-AP (CDK5R2/p39 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 27058-1-AP

    IHC staining of mouse brain using 27058-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27058-1-AP (CDK5R2/p39 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CDK5R2/p39 Polyclonal antibody

CDK5R2/p39 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27058-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CDK5 activator 2, CDK5R2, CDK5R2/p39, NCK5AI, P39, p39I, p39nck5ai

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNeuro-2a cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27058-1-AP targets CDK5R2/p39 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 CDK5R2/p39 fusion protein Ag25834 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase 5, regulatory subunit 2 (p39)
别名 CDK5 activator 2, CDK5R2, CDK5R2/p39, NCK5AI, P39, p39I, p39nck5ai
观测分子量 39 kDa
GenBank蛋白编号BC041771
基因名称 CDK5R2/p39
Gene ID (NCBI) 8941
RRIDAB_2880735
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13319
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK5R2 is a neuron-specific activator of CDK5 kinase. It associates with CDK5 to form an active kinase. This protein and neuron-specific CDK5 activator CDK5R1/p39NCK5A both share limited similarity to cyclins, and thus may define a distinct family of cyclin-dependent kinase activating proteins.

实验方案

Product Specific Protocols
WB protocol for CDK5R2/p39 antibody 27058-1-APDownload protocol
IHC protocol for CDK5R2/p39 antibody 27058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

BMC Cancer

m1A regulator-mediated methylation modification patterns correlated with autophagy to predict the prognosis of hepatocellular carcinoma

Authors - Yingmin Wu
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