验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, K-562 cells, U2OS cells, U-87 MG cells |
| Positive IF/ICC detected in | MDCK cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 8 publications below |
产品信息
24428-1-AP targets CEP135 in WB, IF/ICC, ELISA applications and shows reactivity with human, Canine samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, Canine |
| Cited Reactivity | human, mouse |
| Immunogen | CEP135 fusion protein Ag19122 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | centrosomal protein 135kDa |
| Synonyms | centrosomal protein 135kDa, Centrosomal protein 4, Centrosomal protein of 135 kDa, CEP135, CEP4, KIAA0635 |
| Calculated Molecular Weight | 1140 aa, 134 kDa |
| Observed Molecular Weight | 135 kDa |
| GenBank Accession Number | BC136535 |
| Gene Symbol | CEP135 |
| Gene ID (NCBI) | 9662 |
| RRID | AB_2879543 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q66GS9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP135 is a 135 kDa centrosomal protein that is present in a wide range of organisms. CEP135 is located at the centrosome throughout the cell cycle, and localization is independent of the microtubule network. It distributes throughout the centrosomal area in association with the electron-dense material surrounding centrioles.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP135 antibody 24428-1-AP | Download protocol |
| IF protocol for CEP135 antibody 24428-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Curr Biol SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells. | ||
EMBO Rep ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis. | ||
Elife WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity. | ||
Cells Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells. | ||
J Cell Sci Separation-of-function MCPH-associated mutations in CPAP affect centriole number and length |
