验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14173-1-AP targets CHD4 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | CHD4 fusion protein Ag5372 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromodomain helicase DNA binding protein 4 |
| 别名 | Mi 2b, EC:3.6.4.12, Chromodomain-helicase-DNA-binding protein 4, CHD-4, CHD 4 |
| 计算分子量 | 1937 aa, 221 kDa |
| 观测分子量 | 260 kDa |
| GenBank蛋白编号 | BC038596 |
| 基因名称 | CHD4 |
| Gene ID (NCBI) | 1108 |
| RRID | AB_2080046 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14839 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calculated molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHD4 antibody 14173-1-AP | Download protocol |
| IHC protocol for CHD4 antibody 14173-1-AP | Download protocol |
| IF protocol for CHD4 antibody 14173-1-AP | Download protocol |
| IP protocol for CHD4 antibody 14173-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression. | ||
Leukemia Runx1 repression by histone deacetylation is critical for Setbp1-induced mouse myeloid leukemia development. | ||
Cancer Res CHD4 Promotes Breast Cancer Progression as a Coactivator of Hypoxia-Inducible Factors.
| ||
Arthritis Rheumatol Functional Genomic Analysis of a RUNX3 Polymorphism Associated with Ankylosing Spondylitis. | ||
Clin Epigenetics CHD4/NuRD complex regulates complement gene expression and correlates with CD8 T cell infiltration in human hepatocellular carcinoma.
| ||
Cell Signal CHD4 mediates SOX2 transcription through TRPS1 in luminal breast cancer
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