验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, HEK293 cells, mouse kidney tissue, mouse spleen tissue, mouse thymus tissue, HEK-293 cells, mouse brain tissue, mouse heart tissue, HeLa cells |
| Positive IP detected in | mouse kidney tissue, HeLa cells |
| Positive IHC detected in | human heart tissue, human spleen tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14276-1-AP targets CNOT1 in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP, chIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Immunogen | CNOT1 fusion protein Ag5623 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CCR4-NOT transcription complex, subunit 1 |
| Synonyms | hNOT1, CDC39, CCR4-NOT transcription complex subunit 1, CCR4-associated factor 1, AD-005 |
| Calculated Molecular Weight | 267 kDa |
| Observed Molecular Weight | 240-250 kDa, 174 kDa |
| GenBank Accession Number | BC040523 |
| Gene Symbol | CNOT1 |
| Gene ID (NCBI) | 23019 |
| RRID | AB_10888627 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A5YKK6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms.This is a rabbit polyclonal antibody raised against part chain of C-terminal CNOT1 of human origin. CNOT1 exsits as many isoforms and molecular weight of isoforms are 267, 241 and 173 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNOT1 antibody 14276-1-AP | Download protocol |
| IHC protocol for CNOT1 antibody 14276-1-AP | Download protocol |
| IF protocol for CNOT1 antibody 14276-1-AP | Download protocol |
| IP protocol for CNOT1 antibody 14276-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. | ||
Cell Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs. | ||
Cell The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. | ||
Nat Cancer SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers | ||
Mol Cell An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation | ||
Mol Cell Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation
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