验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14102-1-AP targets CNOT7 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | CNOT7 fusion protein Ag5235 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CCR4-NOT transcription complex, subunit 7 |
| Synonyms | BTG1 binding factor 1, CAF 1, CAF1, CCR4 associated factor 1, CNOT7, hCAF 1 |
| Calculated Molecular Weight | 285 aa, 33 kDa |
| Observed Molecular Weight | 33-35 kDa |
| GenBank Accession Number | BC060852 |
| Gene Symbol | CNOT7 |
| Gene ID (NCBI) | 29883 |
| RRID | AB_2245087 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UIV1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cnot7, Ccr4-Not complex 7, is a component of CCR4-NOT complex and known to be a transcription factor and a modulator of mRNA degradation in yeast. Mammalian Cnot7 interacts with Tob and its family members, which is known as an inhibitor of BMP signaling through interfering with the Smad system and is involved in bone metabolism [PMID:17451368]. It is one of at least 8 subunits that make up the CCR4-Not transcription complex, which is involved in negative regulation of the pol II directed transcription in yeast24 and may be involved inglobal gene regulation by modulation of TATAA-binding protein (TBP) activity [PMID:10880468].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNOT7 antibody 14102-1-AP | Download protocol |
| IP protocol for CNOT7 antibody 14102-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. | ||
Nat Commun TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV. | ||
Open Biol RNA binding protein AUF1/HNRNPD regulates nuclear export, stability and translation of SNCA transcripts | ||
J Inflamm (Lond) The functional characterization of phosphorylation of tristetraprolin at C-terminal NOT1-binding domain.
| ||
Int J Mol Med B cell translocation gene 1 reduces the biological outcome of kidney cancer through induction of cell proliferation, cell cycle arrest, cell apoptosis and cell metastasis. | ||
Cells Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN). |
