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Carboxypeptidase E Polyclonal antibody

Carboxypeptidase E Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13710-1-AP

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Synonyms

Prohormone-processing carboxypeptidase, EC:3.4.17.10, CPH, CPE, Carboxypeptidase H



经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pituitary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13710-1-AP targets Carboxypeptidase E in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Carboxypeptidase E fusion protein Ag4647 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carboxypeptidase E
Synonyms Prohormone-processing carboxypeptidase, EC:3.4.17.10, CPH, CPE, Carboxypeptidase H
Calculated Molecular Weight 476 aa, 53 kDa
Observed Molecular Weight 50 kDa, 52 kDa
GenBank Accession NumberBC033866
Gene Symbol Carboxypeptidase E
Gene ID (NCBI) 1363
RRIDAB_2083913
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16870
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.

实验方案

Product Specific Protocols
WB protocol for Carboxypeptidase E antibody 13710-1-APDownload protocol
IHC protocol for Carboxypeptidase E antibody 13710-1-APDownload protocol
IF protocol for Carboxypeptidase E antibody 13710-1-APDownload protocol
IP protocol for Carboxypeptidase E antibody 13710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Cell Metab

Reductive TCA cycle metabolism fuels glutamine- and glucose-stimulated insulin secretion.

Authors - Guo-Fang Zhang
mouseWB

Diabetes

Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function.

Authors - Seokwon Jo

Am J Physiol Endocrinol Metab

Loss of the Golgi-localized v-ATPase subunit does not alter insulin granule formation or pancreatic islet β-cell function

Authors - Cierra K Boyer
human, mouseIHC

Brain Pathol

Secretory sorting receptors carboxypeptidase E and secretogranin III in amyloid β-associated neural degeneration in Alzheimer's disease.

Authors - Virginia Plá
humanWB,IHC

Front Genet

Development and Validation of a Novel Gene Signature for Predicting the Prognosis by Identifying m5C Modification Subtypes of Cervical Cancer.

Authors - Jing Yu
  • KD Validated
ratWB

Biophys Rep

PMID: 30596141

Authors - Min Li