验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, COLO 320 cells, human brain tissue, human heart tissue, A375 cells, mouse liver tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | COLO 320 cells, A375 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11435-1-AP targets CRBN in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | CRBN fusion protein Ag1951 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cereblon |
| 别名 | cereblon, CRBN, MRT2A, Protein cereblon |
| 计算分子量 | 442 aa, 51 kDa |
| 观测分子量 | 51-55 kDa |
| GenBank蛋白编号 | BC017419 |
| 基因名称 | CRBN |
| Gene ID (NCBI) | 51185 |
| RRID | AB_2085739 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96SW2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRBN plays an important role in assembly and surface expression of functional BK(Ca) channels. The mutation of human cereblon gene (CRBN) is related with mild mental retardation, and causes ARNSID (nonsyndromal intellectual disability) by distubing the developmental regulation of BK(Ca) in brain regions that are critical for memory and learning. CRBN is also identified as important thalidomide-binding protein for limb outgrowth in zebrafish and chicks. It can be detected a band above 51 kDa (maybe 55 kDa) in HT22 cells(PMID: 21232561).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CRBN antibody 11435-1-AP | Download protocol |
| IHC protocol for CRBN antibody 11435-1-AP | Download protocol |
| IF protocol for CRBN antibody 11435-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science DRUG DEVELOPMENT. Phthalimide conjugation as a strategy for in vivo target protein degradation.
| ||
Mol Cell BET Bromodomain Proteins Function as Master Transcription Elongation Factors Independent of CDK9 Recruitment.
| ||
Nat Commun Homo-PROTACs: bivalent small-molecule dimerizers of the VHL E3 ubiquitin ligase to induce self-degradation. | ||
Cell Rep The Drug Vehicle and Solvent N-Methylpyrrolidone Is an Immunomodulator and Antimyeloma Compound. | ||
Elife Targeted degradation of BRD9 reverses oncogenic gene expression in synovial sarcoma. | ||
FASEB J AMPK is down-regulated by the CRL4A-CRBN axis through the polyubiquitination of AMPKα isoforms.
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