验证数据展示
经过测试的应用
| Positive WB detected in | MDA-MB-231 cells, COLO 320 cells, human adrenal gland tissue, human testis tissue, human saliva, SW480 cells, mouse skin tissue |
| Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16025-1-AP targets Cystatin SN/CST1 in WB, IHC, IF, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP, ELISA |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | Cystatin SN/CST1 fusion protein Ag8862 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cystatin SN |
| 别名 | CST1, Cystatin-1, cystatin SN, Cystatin 1, Cystain-SA-I |
| 计算分子量 | 141 aa, 16 kDa |
| 观测分子量 | 14-16 kDa |
| GenBank蛋白编号 | BC021225 |
| 基因名称 | CST1 |
| Gene ID (NCBI) | 1469 |
| RRID | AB_10916385 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P01037 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CST1, or cystatin SN, is a gene in humans that encodes a secreted protein belonging to the type 2 cystatin superfamily, which includes CST1, CST2, CST3, CST4, and CST5. These proteins are cysteine proteinase inhibitors found in various human fluids and secretions, where they appear to provide protective functions . CST1 is specifically found in saliva, tears, urine, and seminal fluid, and it plays a role in inhibiting the activity of cysteine proteases. CST1 has been implicated in various pathological processes, including tumor invasion and metastasis. Overexpression of CST1 has been observed in several types of cancer, such as lung, breast, colorectal, and gastric cancer, suggesting its role in the proliferation, invasion, and metastasis of these tumors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cystatin SN/CST1 antibody 16025-1-AP | Download protocol |
| FC protocol for Cystatin SN/CST1 antibody 16025-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Med Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application | ||
Nat Commun Integrated single-cell transcriptome analysis reveals heterogeneity of esophageal squamous cell carcinoma microenvironment. | ||
Cell Res Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis. | ||
Oncogene CST1 inhibits ferroptosis and promotes gastric cancer metastasis by regulating GPX4 protein stability via OTUB1
| ||
Mol Oncol Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression. | ||
Aging (Albany NY) Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism. |
