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Cathepsin V Polyclonal antibody

Cathepsin V Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18442-1-AP

Print datasheet

Synonyms

CTSL2, CTSV, CTSU, CATL2, Cathepsin U

验证数据展示

  • WB analysis using 18442-1-AP

    WB analysis using 18442-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18442-1-AP (Cathepsin V antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 18442-1-AP

    WB analysis of human testis using 18442-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 18442-1-AP (Cathepsin V antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 18442-1-AP

    IHC staining of human skin cancer using 18442-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18442-1-AP (Cathepsin V antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 18442-1-AP

    IHC staining of human skin cancer using 18442-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18442-1-AP (Cathepsin V antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, human testis tissue, rat liver tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

18442-1-AP targets Cathepsin V in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Cathepsin V fusion protein Ag13284 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cathepsin L2
Synonyms CTSL2, CTSV, CTSU, CATL2, Cathepsin U
Calculated Molecular Weight 37 kDa
Observed Molecular Weight26-36 kDa
GenBank Accession NumberBC023504
Gene Symbol Cathepsin V
Gene ID (NCBI) 1515
RRIDAB_10644340
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60911
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cathepsin V is a lysosomal cysteine protease that has an important role in corneal physiology. Cathepsin V was found to be differentially expressed in relation to pigmentary skin backgrounds, and was about 7.5-folds higher in keratinocytes from lightly pigmented, relative to darkly pigmented skins. It is expressed in primary keratinocytes, melanocytes, and HaCaT keratinocytes, but not in primary fibroblasts. The purified cathepsin L2 was a high molecular weight proteinase of about 78 kDa, but it was unstable to SDS and reducing conditions, which dissociated parts of the proteinase into 66 kDa, 31 kDa and 26 kDa fragments(Food Chemistry,Volume11,Issue4,879-886) and it can form a complex of 70 kDa with an unidentified subunit(PMID:1930136).

实验方案

Product Specific Protocols
WB protocol for Cathepsin V antibody 18442-1-APDownload protocol
IHC protocol for Cathepsin V antibody 18442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Mol Carcinog

Cathepsin V is correlated with the prognosis and tumor microenvironment in liver cancer

Authors - Junyu Liu
View Article