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CYP20A1 Polyclonal antibody

CYP20A1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16702-1-AP

Print datasheet

Synonyms

CYP M, CYP20A1, Cytochrome P450 20A1

验证数据展示

  • WB analysis of HEK-293 using 16702-1-AP

    WB analysis of HEK-293 using 16702-1-AP

    WB result of CYP20A1 antibody (16702-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP20A1 transfected HEK-293 cells.

  • WB analysis using 16702-1-AP

    WB analysis using 16702-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 16702-1-AP

    IHC staining of human liver cancer using 16702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16702-1-AP (CYP20A1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 16702-1-AP

    IHC staining of human liver cancer using 16702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16702-1-AP (CYP20A1 antibody) at dilution of 1:200 (under 40x lens).

  • IP experiment of MCF-7 using 16702-1-AP

    IP experiment of MCF-7 using 16702-1-AP

    IP result of anti-CYP20A1 (IP:16702-1-AP, 4ug; Detection:16702-1-AP 1:300) with MCF-7 cells lysate 2400 ug.

经过测试的应用

Positive WB detected inHeLa cells, mouse lung tissue, HEK-293 cells, K-562 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16702-1-AP targets CYP20A1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CYP20A1 fusion protein Ag10099 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 20, subfamily A, polypeptide 1
Synonyms CYP M, CYP20A1, Cytochrome P450 20A1
Calculated Molecular Weight 462 aa, 52 kDa
Observed Molecular Weight 45-52 kDa
GenBank Accession NumberBC020616
Gene Symbol CYP20A1
Gene ID (NCBI) 57404
RRIDAB_2261395
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UW02
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CYP20A1 antibody 16702-1-APDownload protocol
IHC protocol for CYP20A1 antibody 16702-1-APDownload protocol
IP protocol for CYP20A1 antibody 16702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols