验证数据展示
经过测试的应用
| Positive WB detected in | Tunicamycin treated HepG2 cells, Tunicamycin treated HeLa cells, Staurosporine treated Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25546-1-AP targets cleaved-Caspase 3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, hamster, sheep |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | caspase 3, apoptosis-related cysteine peptidase |
| 别名 | cleaved-Caspase 3 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | NM_032991 |
| 基因名称 | Caspase 3 |
| Gene ID (NCBI) | 836 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P42574 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspase 3, also named CPP32, SCA-1, and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7, and -9. CASP3 is involved in the cleavage of huntingtin. The antibody can recognize active-caspase 3.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for cleaved-Caspase 3 antibody 25546-1-AP | Download protocol |
| IHC protocol for cleaved-Caspase 3 antibody 25546-1-AP | Download protocol |
| IF protocol for cleaved-Caspase 3 antibody 25546-1-AP | Download protocol |
| IP protocol for cleaved-Caspase 3 antibody 25546-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
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