• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Cyclin H Monoclonal antibody

Cyclin H Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2C5G11

Cat no : 67065-1-Ig

Print datasheet

Synonyms

CCNH, 2C5G11, p34, MO15-associated protein, MO15 associated protein

验证数据展示

  • WB analysis using 67065-1-Ig

    WB analysis using 67065-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67065-1-Ig (Cyclin H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67065-1-Ig

    IHC staining of human breast cancer using 67065-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67065-1-Ig (Cyclin H antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67065-1-Ig

    IHC staining of human breast cancer using 67065-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67065-1-Ig (Cyclin H antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 67065-1-Ig

    IF Staining of PC-3 using 67065-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using Cyclin H antibody (67065-1-Ig, Clone: 2C5G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of PC-3 using 67065-1-Ig

    FC experiment of PC-3 using 67065-1-Ig

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug Anti-Human Cyclin H (67065-1-Ig, Clone:2C5G11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, MCF-7 cells, NCCIT cells, HT-1080 cells, LNCaP cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells
Positive FC (Intra) detected inPC-3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67065-1-Ig targets Cyclin H in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Cyclin H fusion protein Ag28556 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name cyclin H
Synonyms CCNH, 2C5G11, p34, MO15-associated protein, MO15 associated protein
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC005280
Gene Symbol Cyclin H
Gene ID (NCBI) 902
RRIDAB_2882375
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51946
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Cyclin H antibody 67065-1-IgDownload protocol
IHC protocol for Cyclin H antibody 67065-1-IgDownload protocol
IF protocol for Cyclin H antibody 67065-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Genes Dev

TRF1 uses a noncanonical function of TFIIH to promote telomere replication

Authors - Zhe Yang
View Article