验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy DAPK1 Ready-To-Use IHC Kit. DAPK1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25136-1-AP targets DAPK1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, goat |
| 免疫原 | DAPK1 fusion protein Ag18387 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | death-associated protein kinase 1 |
| 别名 | DAP kinase 1, DAPK, DAPK1 |
| 计算分子量 | 1430 aa, 160 kDa |
| 观测分子量 | 140-160 kDa |
| GenBank蛋白编号 | BC113660 |
| 基因名称 | DAPK1 |
| Gene ID (NCBI) | 1612 |
| RRID | AB_2879917 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P53355 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DAPK1(Death-associated protein kinase 1) is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. Loss of DAPK1 expression is associated with a selective advantage fortumor cells to resist apoptotic stimuli, allowing them to separate from the original tumor; from this point of view, DAPK1 could be considered a tumor metastases inhibitor gene(PMID:17319784). This protein has 3 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DAPK1 antibody 25136-1-AP | Download protocol |
| IHC protocol for DAPK1 antibody 25136-1-AP | Download protocol |
| IF protocol for DAPK1 antibody 25136-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Life Sci Auto- and paracrine rewiring of NIX-mediated mitophagy by insulin-like growth factor-binding protein 7 in septic AKI escalates inflammation-coupling tubular damage | ||
Aging (Albany NY) Low DAPK1 expression correlates with poor prognosis and sunitinib resistance in clear cell renal cell carcinoma. | ||
Stem Cell Res Ther Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.
| ||
J Alzheimers Dis Oxytocin Alleviates MPTP-Induced Neurotoxicity in Mice by Targeting MicroRNA-26a/Death-Associated Protein Kinase 1 Pathway. | ||
Stem Cells Dev Reduced DAPK1 Expression Promotes Stem Cell-Like Characteristics of Prostate Cancer Cells by Activating ZEB1 via Hippo/YAP Signaling Pathway.
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