验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13873-1-AP targets DGKH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | DGKH fusion protein Ag4926 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | diacylglycerol kinase, eta |
| 别名 | EC:2.7.1.107, DGK-eta, DGKeta, DAG kinase eta |
| 计算分子量 | 1100 aa, 122 kDa |
| 观测分子量 | 135 kDa |
| GenBank蛋白编号 | BC043292 |
| 基因名称 | DGKH |
| Gene ID (NCBI) | 160851 |
| RRID | AB_2091287 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86XP1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DGKH(Diacylglycerol kinase eta) is also named as DGK-eta and belongs to the eukaryotic diacylglycerol kinase family. It is a key protein in the lithium-sensitive phosphatidyl inositol pathway, and recently a link between environmental stress and schizophrenia/bipolar disorder has been revealed. DGKH has some isoforms produced by alternative splicing. Two isoforms DGKH1 and DGKH2 with the molecular weight of 128 kDa and 135 kDa shows diacylglycerol kinase activity; however, the 2 isoforms differed in several other characteristics(PMID:12810723).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DGKH antibody 13873-1-AP | Download protocol |
| IHC protocol for DGKH antibody 13873-1-AP | Download protocol |
| IF protocol for DGKH antibody 13873-1-AP | Download protocol |
| IP protocol for DGKH antibody 13873-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Invest Dermatol Diacylglycerol kinase regulates tyrosinase expression and function in human melanocytes. | ||
BMC Dev Biol Distinct expression and localization of the type II diacylglycerol kinase isozymes ¿, ¿ and ¿ in the mouse reproductive organs. | ||
J Histochem Cytochem Expression and Localization of Type II Diacylglycerol Kinase Isozymes δ and η in the Developing Mouse Brain. | ||
Clin Transl Oncol Diacylglycerol kinase η modulates oncogenic properties of lung cancer cells.
| ||
Biochim Biophys Acta Mol Cell Biol Lipids Myristic acid specifically stabilizes diacylglycerol kinase δ protein in C2C12 skeletal muscle cells. | ||
Cancer Med Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma |
