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验证数据展示

  • WB analysis of mouse kidney using 16314-1-AP

    WB analysis of mouse kidney using 16314-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16314-1-AP (dGK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 16314-1-AP

    IHC staining of human hepatocirrhosis using 16314-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16314-1-AP (dGK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human hepatocirrhosis using 16314-1-AP

    IHC staining of human hepatocirrhosis using 16314-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16314-1-AP (dGK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human hepatocirrhosis using 16314-1-AP

    IHC staining of human hepatocirrhosis using 16314-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16314-1-AP (dGK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human hepatocirrhosis using 16314-1-AP

    IHC staining of human hepatocirrhosis using 16314-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16314-1-AP (dGK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

dGK Polyclonal antibody

dGK Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16314-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

DGUOK, EC:2.7.1.76, EC:2.7.1.113, Deoxyguanosine kinase, Deoxyadenosine kinase, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16314-1-AP targets dGK in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 dGK fusion protein Ag9361 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deoxyguanosine kinase
别名 DGUOK, EC:2.7.1.76, EC:2.7.1.113, Deoxyguanosine kinase, Deoxyadenosine kinase, mitochondrial
计算分子量 277 aa, 32 kDa
观测分子量 28-32 kDa, 56-64 kDa
GenBank蛋白编号BC015757
基因名称 DGUOK
Gene ID (NCBI) 1716
RRIDAB_10638311
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16854
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Deoxyguanosine kinase (dGK) deficiency is a frequent cause of mitochondrial DNA depletion associated with a hepatocerebral phenotype. dGK is a member of the deoxyribonucleoside kinase family involved in phosphorylation of deoxyribonucleosides. In mammals, there are four deoxyribonucleoside kinases with overlapping specificities: two cytoplasmic enzymes, thymidine kinase 1 (TK1) and deoxycytidine kinase (dCK), and two mitochondrial enzymes, thymidine kinase 2 (TK2) and deoxyguanosine kinase (dGK). The human dGK protein is highly specific for purine substrates. Human dGK is a dimer of two identical subunits of 30 kDa. The three-dimensional structure of the human dGK monomer has been reported, providing an explanation for the substrate specificity of the enzyme. (PMID: 29786478, PMID: 11427893, PMID: 17073823)

实验方案

Product Specific Protocols
WB protocol for dGK antibody 16314-1-APDownload protocol
IHC protocol for dGK antibody 16314-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols