验证数据展示
经过测试的应用
Positive WB detected in | A2780 cells, MCF-7 cells, SKOV-3 cells, mouse heart tissue, mouse ovary tissue, mouse spleen tissue, rat spleen tissue |
Positive IP detected in | mouse spleen tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse kidney tissue |
Positive FC (Intra) detected in | HEK-293T cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14877-1-AP targets DHODH in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF-P, FC (Intra), IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, chicken, goat |
免疫原 | DHODH fusion protein Ag6649 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | dihydroorotate dehydrogenase |
别名 | PYRD, EC:1.3.5.2, Dihydroorotate oxidase, Dihydroorotate dehydrogenase (quinone), mitochondrial, dihydroorotate dehydrogenase |
计算分子量 | 43 kDa |
观测分子量 | 43 kDa |
GenBank蛋白编号 | BC065245 |
基因名称 | DHODH |
Gene ID (NCBI) | 1723 |
RRID | AB_2091723 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q02127 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).
实验方案
Product Specific Protocols | |
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WB protocol for DHODH antibody 14877-1-AP | Download protocol |
IHC protocol for DHODH antibody 14877-1-AP | Download protocol |
IF protocol for DHODH antibody 14877-1-AP | Download protocol |
IP protocol for DHODH antibody 14877-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression | ||
Nature DHODH-mediated ferroptosis defence is a targetable vulnerability in cancer.
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Science Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1. | ||
Cancer Discov PTEN Regulates Glutamine Flux to Pyrimidine Synthesis and Sensitivity to Dihydroorotate Dehydrogenase Inhibition. | ||
Cancer Cell Cancer-selective metabolic vulnerabilities in MYC-amplified medulloblastoma
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Cell Metab Macrophage-Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer.
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