验证数据展示

WB analysis of HeLa using 20567-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20567-1-AP (DICER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20567-1-AP
WB result of DICER1 antibody (20567-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-DICER1 transfected HepG2 cells.
IP experiment of Jurkat using 20567-1-AP
IP result of anti-DICER1 (IP:20567-1-AP, 5ug; Detection:20567-1-AP 1:300) with Jurkat cells lysate 2000ug.
IHC staining of human testis using 20567-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of human ovary tumor using 20567-1-AP
Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using DICER1 antibody (20567-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human ovary tumor using 20567-1-AP
Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using DICER1 antibody (20567-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 20567-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DICER1 antibody (20567-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HepG2 using 20567-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human DICER1 (20567-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

DICER1 Polyclonal antibody

DICER1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 20567-1-AP

Publications(24)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (1)

Synonyms

HERNA, Helicase MOI, Endoribonuclease Dicer, EC:3.1.26.3, DICER

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size:

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概述

经过测试的应用
产品信息
发表文章 (24)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, K-562 cells, HepG2 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human testis tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human ovary tumor tissue
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 22 publications below
IHC	See 1 publications below
IF	See 3 publications below
IP	See 2 publications below

产品信息

20567-1-AP targets DICER1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Applications	WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP
Tested Reactivity	human
Cited Reactivity	human
Immunogen	Peptide 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	dicer 1, ribonuclease type III
Synonyms	HERNA, Helicase MOI, Endoribonuclease Dicer, EC:3.1.26.3, DICER
Calculated Molecular Weight	219 kDa
Observed Molecular Weight	220-250 kDa, 90 kDa
GenBank Accession Number	NM_030621
Gene Symbol	DICER1
Gene ID (NCBI)	23405
RRID	AB_10694287
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UPY3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

DICER1, also named as DICER, HERNA and KIAA0928, belongs to the helicase family and Dicer subfamily. It is required for formation of the RNA induced silencing complex (RISC). DICER1 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. DICER1 cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs. The antibody is specific to DICER1. The calcualted molecular weight of DICER1 is 219 kDa, but modified DICER1 is about 220-250 kDa. DICER1 exists some isoforms with molecular weight 219 kDa and 93 kDa.

实验方案

Product Specific Protocols
WB protocol for DICER1 antibody 20567-1-AP	Download protocol
IHC protocol for DICER1 antibody 20567-1-AP	Download protocol
IF protocol for DICER1 antibody 20567-1-AP	Download protocol
IP protocol for DICER1 antibody 20567-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
	WB	Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. Authors - Xiaohui Li KD Validated View Article
human	WB	Nat Chem Biol Differential roles of human PUS10 in miRNA processing and tRNA pseudouridylation. Authors - Jinghui Song View Article
human	WB	Nat Commun RNA structure probing reveals the structural basis of Dicer binding and cleavage. Authors - Qing-Jun Luo View Article
human	WB,IP	Nucleic Acids Res HSP90 and Aha1 modulate microRNA maturation through promoting the folding of Dicer1. Authors - Xiaochuan Liu View Article
human	WB	Nat Commun Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling. Authors - Chen Xu View Article
human	WB	J Pathol Cancer-associated somatic DICER1 hotspot mutations cause defective miRNA processing and reverse-strand expression bias to predominantly mature 3p strands through loss of 5p strand cleavage. Authors - Anglesio M S MS KO Validated View Article

验证数据展示

WB analysis of HeLa using 20567-1-AP

WB analysis of HepG2 using 20567-1-AP

IP experiment of Jurkat using 20567-1-AP

IHC staining of human testis using 20567-1-AP

IF Staining of human ovary tumor using 20567-1-AP

IF Staining of human ovary tumor using 20567-1-AP

IF Staining of HepG2 using 20567-1-AP

FC experiment of HepG2 using 20567-1-AP

DICER1 Polyclonal antibody

DICER1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

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经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Differential roles of human PUS10 in miRNA processing and tRNA pseudouridylation.

RNA structure probing reveals the structural basis of Dicer binding and cleavage.

HSP90 and Aha1 modulate microRNA maturation through promoting the folding of Dicer1.

Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling.

Cancer-associated somatic DICER1 hotspot mutations cause defective miRNA processing and reverse-strand expression bias to predominantly mature 3p strands through loss of 5p strand cleavage.