验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, HeLa cells, human testis tissue, rat testis tissue |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy DMC1 Ready-To-Use IHC Kit. DMC1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)-P | IF-P : 1:600-1:2400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13714-1-AP targets DMC1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | DMC1 fusion protein Ag4653 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) |
| 别名 | DMC1H, HsLim15, LIM15 |
| 计算分子量 | 165 aa, 18 kDa, 38 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC035658 |
| 基因名称 | DMC1 |
| Gene ID (NCBI) | 11144 |
| RRID | AB_2091076 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14565 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DMC1 antibody 13714-1-AP | Download protocol |
| IF protocol for DMC1 antibody 13714-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells. | ||
Nucleic Acids Res MEIOK21: a new component of meiotic recombination bridges required for spermatogenesis. | ||
Cell Prolif ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progression | ||
Cell Death Differ NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis. | ||
Elife A missense in HSF2BP causing primary ovarian insufficiency affects meiotic recombination by its novel interactor C19ORF57/BRME1. | ||
Front Oncol The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL). |
