验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, human testis tissue, HepG2 cells, human liver tissue, Jurkat cells, mouse lung tissue, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy DNAJA1 Ready-To-Use IHC Kit. DNAJA1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11713-1-AP targets DNAJA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | DNAJA1 fusion protein Ag2284 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | DnaJ (Hsp40) homolog, subfamily A, member 1 |
| Synonyms | hDj-2, HDJ2, hDJ 2, DNAJ2, DjA1 |
| Calculated Molecular Weight | 397 aa, 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC008182 |
| Gene Symbol | DNAJA1 |
| Gene ID (NCBI) | 3301 |
| RRID | AB_10637266 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P31689 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJA1 antibody 11713-1-AP | Download protocol |
| IHC protocol for DNAJA1 antibody 11713-1-AP | Download protocol |
| IF protocol for DNAJA1 antibody 11713-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition | ||
Nat Commun Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells. | ||
J Clin Invest Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.
| ||
Mol Ther Nucleic Acids Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction | ||
Sci Rep Co-chaperones DNAJA1 and DNAJB6 are critical for regulation of polyglutamine aggregation.
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