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CRMP2 Polyclonal antibody

CRMP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14521-1-AP

Print datasheet

Synonyms

CRMP 2, CRMP2, DHPRP2, dihydropyrimidinase like 2, DPYSL2, DRP 2, DRP2, N2A3, ULIP 2, ULIP2, Unc 33 like phosphoprotein 2

验证数据展示

  • WB analysis using 14521-1-AP

    WB analysis using 14521-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14521-1-AP (CRMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 14521-1-AP

    IHC staining of human colon cancer using 14521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14521-1-AP (CRMP2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DPYSL2 antibody included
  • IHC staining of human colon cancer using 14521-1-AP

    IHC staining of human colon cancer using 14521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14521-1-AP (CRMP2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DPYSL2 antibody included

经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DPYSL2 Ready-To-Use IHC Kit. DPYSL2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14521-1-AP targets CRMP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CRMP2 fusion protein Ag5987 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dihydropyrimidinase-like 2
Synonyms CRMP 2, CRMP2, DHPRP2, dihydropyrimidinase like 2, DPYSL2, DRP 2, DRP2, N2A3, ULIP 2, ULIP2, Unc 33 like phosphoprotein 2
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC067109
Gene Symbol CRMP2
Gene ID (NCBI) 1808
RRIDAB_2094341
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16555
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dihydropyrimidinase-like 2 (Dpysl2), a protein involved in neuronal differentiation and axonal guidance is found altered in various nervous system diseases (PMID:19524114). It is also named as CRMP2, ULIP2 and belongs to hydantoinase/dihydropyrimidinase subfamily. The phosphorylation of the particular portion carboxyl-terminal to the basic region of DPYSL2 may play an important role in regulating its activity, and may be involved in the formation of degenerating neurites in AD brain(PMID:10757975).

实验方案

Product Specific Protocols
WB protocol for CRMP2 antibody 14521-1-APDownload protocol
IHC protocol for CRMP2 antibody 14521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Neural Regen Res

GIT1 enhances neurite outgrowth by stimulating microtubule assembly.

Authors - Yi-Sheng Li
View Article