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验证数据展示

  • IHC staining of human lung cancer using 51005-2-AP

    IHC staining of human lung cancer using 51005-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51005-2-AP (DUSP22 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 51005-2-AP

    IHC staining of human lung cancer using 51005-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51005-2-AP (DUSP22 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HEK-293 using 51005-2-AP

    WB analysis of HEK-293 using 51005-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 51005-2-AP (DUSP22 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

DUSP22 Polyclonal antibody

DUSP22 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 51005-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

DUSP22, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51005-2-AP targets DUSP22 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dual specificity phosphatase 22
别名 DUSP22, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX
计算分子量 184 aa, 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC022847
基因名称 DUSP22
Gene ID (NCBI) 56940
RRIDAB_2230783
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRW4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DUSP22 antibody 51005-2-APDownload protocol
IHC protocol for DUSP22 antibody 51005-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols