验证数据展示
经过测试的应用
| Positive WB detected in | rat heart tissue, mouse heart tissue, mouse kidney tissue |
| Positive IHC detected in | human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy DUSP7 Ready-To-Use IHC Kit. DUSP7 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
26910-1-AP targets DUSP7/PYST2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | DUSP7/PYST2 fusion protein Ag25488 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | dual specificity phosphatase 7 |
| Synonyms | dual specificity phosphatase 7, DUSP7, MKP X, MKPX, PYST2 |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC104880 |
| Gene Symbol | DUSP7 |
| Gene ID (NCBI) | 1849 |
| RRID | AB_2880681 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16829 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human dual-specificity phosphatase 7 (DUSP7/PYST2) is a member of a structurally homologous subfamily of MAP kinase phosphatases. DUSP7 is overexpressed in myeloid leukemia and other malignancies. It is known to bind and dephosphorylate ErkII, and as it, along with the other members of the DUSP family expresses high selectively for MAP kinases. It has been suggested that it functions as a method for selectively activating/deactivating different members of that family.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DUSP7/PYST2 antibody 26910-1-AP | Download protocol |
| IHC protocol for DUSP7/PYST2 antibody 26910-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Transl Med Loss of SETD2 aggravates colorectal cancer progression caused by SMAD4 deletion through the RAS/ERK signalling pathway | ||
Mol Cancer Linc-RoR promotes MAPK/ERK signaling and confers estrogen-independent growth of breast cancer. | ||
Br J Pharmacol L-Hcy-induced cathepsin V mediates the vascular endothelial inflammation in hyperhomocysteinemia. | ||
Addict Biol Dual-specificity phosphatase 15 (DUSP15) in the nucleus accumbens is a novel negative regulator of morphine-associated contextual memory. | ||
Genes Cells Downregulation of DUSP6, a negative regulator of oncogenic ERK signaling, by ACA-28 induces apoptosis in NIH/3T3 cells overexpressing HER2/ErbB2. |
