验证数据展示
经过测试的应用
| Positive WB detected in | rat heart tissue, mouse heart tissue, mouse kidney tissue |
| Positive IHC detected in | human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy DUSP7 Ready-To-Use IHC Kit. DUSP7 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
26910-1-AP targets DUSP7/PYST2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | DUSP7/PYST2 fusion protein Ag25488 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dual specificity phosphatase 7 |
| 别名 | dual specificity phosphatase 7, DUSP7, MKP X, MKPX, PYST2 |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC104880 |
| 基因名称 | DUSP7 |
| Gene ID (NCBI) | 1849 |
| RRID | AB_2880681 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16829 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human dual-specificity phosphatase 7 (DUSP7/PYST2) is a member of a structurally homologous subfamily of MAP kinase phosphatases. DUSP7 is overexpressed in myeloid leukemia and other malignancies. It is known to bind and dephosphorylate ErkII, and as it, along with the other members of the DUSP family expresses high selectively for MAP kinases. It has been suggested that it functions as a method for selectively activating/deactivating different members of that family.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DUSP7/PYST2 antibody 26910-1-AP | Download protocol |
| IHC protocol for DUSP7/PYST2 antibody 26910-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Transl Med Loss of SETD2 aggravates colorectal cancer progression caused by SMAD4 deletion through the RAS/ERK signalling pathway | ||
Mol Cancer Linc-RoR promotes MAPK/ERK signaling and confers estrogen-independent growth of breast cancer. | ||
Br J Pharmacol L-Hcy-induced cathepsin V mediates the vascular endothelial inflammation in hyperhomocysteinemia. | ||
Addict Biol Dual-specificity phosphatase 15 (DUSP15) in the nucleus accumbens is a novel negative regulator of morphine-associated contextual memory. | ||
Genes Cells Downregulation of DUSP6, a negative regulator of oncogenic ERK signaling, by ACA-28 induces apoptosis in NIH/3T3 cells overexpressing HER2/ErbB2. |
