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验证数据展示

  • WB analysis of HeLa using 51105-1-AP

    WB analysis of HeLa using 51105-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 51105-1-AP (HER2/ErbB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 51105-1-AP

    IHC staining of human breast cancer using 51105-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 51105-1-AP (HER2/ErbB2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human breast cancer using 51105-1-AP

    IHC staining of human breast cancer using 51105-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 51105-1-AP (HER2/ErbB2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human breast cancer using 51105-1-AP

    IHC staining of human breast cancer using 51105-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 51105-1-AP (HER2/ErbB2 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 51105-1-AP

    IF Staining of HeLa using 51105-1-AP

    Immunofluorescent analysis of HeLa cells using 51105-1-AP (HER2/ErbB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of SK-BR-3 using 51105-1-AP

    IP experiment of SK-BR-3 using 51105-1-AP

    IP result of anti-HER2/ErbB2 (IP:51105-1-AP, 4ug; Detection:51105-1-AP 1:500) with SK-BR-3 cells lysate 1280 ug.

HER2/ErbB2 Polyclonal antibody

HER2/ErbB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 51105-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CD340, ERBB2, HER 2, HER 2/neu, HER2, MLN 19, MLN19, NEU, NGL, p185, p185erbB2, Proto oncogene c ErbB 2, Proto oncogene Neu, TKR1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inSK-BR-3 cells
Positive IHC detected inhuman breast cancer tissue, Breast Cancer slides
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51105-1-AP targets HER2/ErbB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Synonyms CD340, ERBB2, HER 2, HER 2/neu, HER2, MLN 19, MLN19, NEU, NGL, p185, p185erbB2, Proto oncogene c ErbB 2, Proto oncogene Neu, TKR1
Calculated Molecular Weight 185 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC167147
Gene Symbol HER2/ErbB2
Gene ID (NCBI) 2064
RRIDAB_2099262
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04626
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the EGF receptor family of receptor tyrosine kinases. It has no ligand binding domain of its own. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.

实验方案

Product Specific Protocols
WB protocol for HER2/ErbB2 antibody 51105-1-APDownload protocol
IHC protocol for HER2/ErbB2 antibody 51105-1-APDownload protocol
IF protocol for HER2/ErbB2 antibody 51105-1-APDownload protocol
IP protocol for HER2/ErbB2 antibody 51105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols