Exo70 Polyclonal antibody

Exo70 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 12014-1-AP

Print datasheet

Synonyms

2 5 3p, EX070, Exo70, Exo70p, EXOC1, EXOC7, exocyst complex component 7, KIAA1067, YJL085W



经过测试的应用

Positive WB detected inhuman brain tissue, HEK-293 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12014-1-AP targets Exo70 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Exo70 fusion protein Ag2628 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name exocyst complex component 7
Synonyms 2 5 3p, EX070, Exo70, Exo70p, EXOC1, EXOC7, exocyst complex component 7, KIAA1067, YJL085W
Calculated Molecular Weight 735 aa, 83 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC011045
Gene Symbol Exo70
Gene ID (NCBI) 23265
RRIDAB_2101698
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPT5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Exo70 antibody 12014-1-APDownload protocol
IHC protocol for Exo70 antibody 12014-1-APDownload protocol
IP protocol for Exo70 antibody 12014-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Blood

vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.

Authors - Anish Vaibhav Sharda
mouseWB

Elife

Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis

Authors - Kunli Zhao
ratIF

Mol Neurobiol

The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons.

Authors - Matías Lira
humanWB,IF

Placenta

Exocyst complex protein expression in the human placenta.

Authors - I M Gonzalez
mouseWB,IF,CoIP

Biol Res

Exo70 intracellular redistribution after repeated mild traumatic brain injury.

Authors - Matías Lira
human,ratIF,CoIP

Life Sci Alliance

An isoform-specific function of Cdc42 in regulating mammalian Exo70 during axon formation

Authors - Priyadarshini Ravindran