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验证数据展示

  • WB analysis of human plasma using 66722-1-Ig

    WB analysis of human plasma using 66722-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66722-1-Ig (F8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Factor VIII Monoclonal antibody

Factor VIII Monoclonal Antibody for WB, ELISA
Cat No. 66722-1-Ig

产品说明书

CloneNo. 1C11E11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, ELISA

别名

F8, DXS1253E, Coagulation factor VIII, Antihemophilic factor, AHF

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue, human blood tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66722-1-Ig targets Factor VIII in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Factor VIII fusion protein Ag15772 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 coagulation factor VIII, procoagulant component
别名 F8, DXS1253E, Coagulation factor VIII, Antihemophilic factor, AHF
计算分子量 2351 aa, 267 kDa
观测分子量 70 kDa
GenBank蛋白编号BC022513
基因名称 F8
Gene ID (NCBI) 2157
RRIDAB_2882073
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP00451
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

F8, also named as AHF and F8C, belongs to the multicopper oxidase family. F8, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. F8 can be cleaved to 4 chains: F8a heavy chain, 200 kDa isoform, F8a heavy chain, 92 kDa isoform, F8 B chain and F8a light chain. This antibody can recognize the light chain.

实验方案

Product Specific Protocols
WB protocol for Factor VIII antibody 66722-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols