FCGR2A / CD32a Polyclonal antibody

FCGR2A / CD32a Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15625-1-AP

Print datasheet

Synonyms

CD32, Fc-gamma RII-a, FCG2, Fc gamma RIIa, Fc gamma RII a



经过测试的应用

Positive WB detected inK-562 cells, U-937 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy FCGR2A/CD32A Ready-To-Use IHC Kit. FCGR2A/CD32A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15625-1-AP targets FCGR2A / CD32a in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen FCGR2A / CD32a fusion protein Ag8072 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fc fragment of IgG, low affinity IIa, receptor (CD32)
Synonyms CD32, Fc-gamma RII-a, FCG2, Fc gamma RIIa, Fc gamma RII a
Calculated Molecular Weight 35 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberBC020823
Gene Symbol CD32a
Gene ID (NCBI) 2212
RRIDAB_2246912
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

实验方案

Product Specific Protocols
WB protocol for FCGR2A / CD32a antibody 15625-1-APDownload protocol
IHC protocol for FCGR2A / CD32a antibody 15625-1-APDownload protocol
IF protocol for FCGR2A / CD32a antibody 15625-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,ratWB

Int J Mol Sci

Ursolic Acid Alleviates Neuroinflammation after Intracerebral Hemorrhage by Mediating Microglial Pyroptosis via the NF-κB/NLRP3/GSDMD Pathway

Authors - Pan Lei
ratWB

Exp Neurol

Photobiomodulation therapy promotes neurogenesis by improving post-stroke local microenvironment and stimulating neuroprogenitor cells.

Authors - Luodan Yang
humanWB

Am J Pathol

FcγRIIa ligation induces platelet hypersensitivity to thrombotic stimuli.

Authors - Berlacher Mark D MD
ratWB

J Alzheimers Dis

Treadmill Exercise Exerts Neuroprotection and Regulates Microglial Polarization and Oxidative Stress in a Streptozotocin-Induced Rat Model of Sporadic Alzheimer's Disease.

Authors - Yujiao Lu
mouseWB

Cell Death Dis

Pentraxin 3 regulated by miR-224-5p modulates macrophage reprogramming and exacerbates osteoarthritis associated synovitis by targeting CD32.

Authors - Jianbin Yin
mouseWB,IF

Int Immunopharmacol

Grossamide attenuates inflammation by balancing macrophage polarization through metabolic reprogramming of macrophages in mice

Authors - Wenlong Zhao