验证数据展示
经过测试的应用
| Positive IHC detected in | human lung cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
26272-1-AP targets FGF-21 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, IF-P, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | FGF-21 fusion protein Ag24064 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fibroblast growth factor 21 |
| 别名 | FGF21, UNQ3115/PRO10196, Fibroblast growth factor 21, FGF 21 |
| 计算分子量 | 22 kDa |
| GenBank蛋白编号 | BC018404 |
| 基因名称 | FGF21 |
| Gene ID (NCBI) | 26291 |
| RRID | AB_2880455 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NSA1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibroblast growth factor 21 (FGF21), a member of the FGF superfamily, has emerged as an important metabolic regulator. FGF21 is produced in the liver, adipocytes and skeletal muscle and regulates glucose, energy and lipid metabolism. FGF21 is a stress-responsive hepatokine which is induced in the liver as a response to injury, regeneration, hepatocarcinogenesis and during kidney dysfunction. FGF21 is primarily metabolized in the kidney and renal secretion is the major route of FGF21 elimination. Its plasma levels increase according to the decline in renal function in patients with chronic and acute renal dysfunction.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for FGF-21 antibody 26272-1-AP | Download protocol |
| IF protocol for FGF-21 antibody 26272-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury | ||
Biomed Pharmacother Dihydroartemisinin inhibits ER stress-mediated mitochondrial pathway to attenuate hepatocyte lipoapoptosis via blocking the activation of the PI3K/Akt pathway. | ||
Cell Death Dis FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival. | ||
Sci Rep Brown adipose tissue-derived FGF21 mediates the cardioprotection of dexmedetomidine in myocardial ischemia/reperfusion injury |
