DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein and More (1)

Applications

WB, IF/ICC, IP, ELISA and More (4)

Conjugate

Unconjugated

CloneNo.

2B3C4

Cat no : 66008-3-Ig

Print datasheet

Synonyms

DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag



经过测试的应用

Positive WB detected inTransfected HEK-293 cells
Positive IP detected inTransfected HEK-293 cells
Positive IF/ICC detected inTransfected HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66008-3-Ig targets DYKDDDDK tag in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, RIP, ELISA
Tested Reactivity recombinant protein
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DYKDDDDK tag
Synonyms DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag
Gene Symbol
Gene ID (NCBI)
RRIDAB_2749837
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

实验方案

Product Specific Protocols
WB protocol for DYKDDDDK tag antibody 66008-3-IgDownload protocol
IF protocol for DYKDDDDK tag antibody 66008-3-IgDownload protocol
IP protocol for DYKDDDDK tag antibody 66008-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nature

DHODH-mediated ferroptosis defence is a targetable vulnerability in cancer.

Authors - Chao Mao
WB

Science

Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.

Authors - Qiang Wang
IF

Nat Biomed Eng

Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting guide RNA prevents wet age-related macular degeneration in mice.

Authors - Sikai Ling
WB

Nat Commun

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Authors - Xiaohua Xu
WB,IF

Nat Commun

ID1 expressing macrophages support cancer cell stemness and limit CD8+ T cell infiltration in colorectal cancer

Authors - Shuang Shang
WB

Mol Cell

SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation

Authors - Wenxin Zhang