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  • KD/KO Validated

GABARAP Polyclonal antibody

GABARAP Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

FC, IF, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 18723-1-AP

Print datasheet

Synonyms

FLC3B, GABARAP, MM46



经过测试的应用

Positive WB detected inHeLa cells, A549 cells, mouse brain tissue, human placenta tissue, mouse pancreas tissue, human heart tissue, mouse skeletal muscle tissue, HuH-7 cells, SH-SY5Y cells, RAW 264.7 cells, mouse liver tissue, rat brain tissue
Positive IF detected inHepG2 cells, Starvation treated HepG2 cells
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18723-1-AP targets GABARAP in WB, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IF, WB, ELISA
Cited Applications CoIP, IF, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, monkey
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GABA(A) receptor-associated protein
Synonyms FLC3B, GABARAP, MM46
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14-18 kDa
GenBank Accession NumberBC106748
Gene Symbol GABARAP
Gene ID (NCBI) 11337
RRIDAB_10603365
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95166
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.

实验方案

Product Specific Protocols
WB protocol for GABARAP antibody 18723-1-APDownload protocol
IF protocol for GABARAP antibody 18723-1-APDownload protocol
FC protocol for GABARAP antibody 18723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.

Authors - Linyu Sun
humanWB

Autophagy

ATF3 -activated accelerating effect of LINC00941/lncIAPF on fibroblast-to-myofibroblast differentiation by blocking autophagy depending on ELAVL1/HuR in pulmonary fibrosis

Authors - Jinjin Zhang
  • KD Validated
humanWB

Autophagy

ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1

Authors - Weihong Xie
  • KO Validated
IF

Nat Commun

PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection.

Authors - Shifan Wang
humanWB

Autophagy

ATF4 links ER stress with reticulophagy in glioblastoma cells.

Authors - Svenja Zielke
mouseWB

Autophagy

ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8.

Authors - Mads Skytte Rasmussen