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验证数据展示

  • WB analysis using 17746-1-AP

    WB analysis using 17746-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17746-1-AP (GBP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of A431 using 17746-1-AP

    IP experiment of A431 using 17746-1-AP

    IP result of anti-GBP4 (IP:17746-1-AP, 4ug; Detection:17746-1-AP 1:500) with A431 cells lysate 1040 ug.

GBP4 Polyclonal antibody

GBP4 Polyclonal Antibody for WB, IP, ELISA
Cat No. 17746-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

Mpa2, Guanylate-binding protein 4, Guanine nucleotide-binding protein 4, GTP-binding protein 4, GBP-4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue, A431 cells, mouse heart tissue
Positive IP detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17746-1-AP targets GBP4 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen GBP4 fusion protein Ag12006 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanylate binding protein 4
Synonyms Mpa2, Guanylate-binding protein 4, Guanine nucleotide-binding protein 4, GTP-binding protein 4, GBP-4
Calculated Molecular Weight 640 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC070055
Gene Symbol GBP4
Gene ID (NCBI) 115361
RRIDAB_2109346
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96PP9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanylate-binding protein 4 (GBP4) is an IFN-inducible GTPase that belongs to the GB1/RHD3 GTPase family. GBP4 plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. GBP4 negatively regulates the antiviral response by inhibiting activation of IRF7 transcription factor. The molecular weight of GBP4 is 73 kDa.

实验方案

Product Specific Protocols
WB protocol for GBP4 antibody 17746-1-APDownload protocol
IP protocol for GBP4 antibody 17746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

mBio

Human GBP1 facilitates the rupture of the Legionella-containing vacuole and inflammasome activation

Authors - Antonia R Bass
View Article