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GCK Polyclonal antibody

GCK Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 19666-1-AP

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Synonyms

GCK, GK, GLK, Glucokinase, glucokinase (hexokinase 4), Hexokinase 4, Hexokinase D, Hexokinase type IV, HHF3, HK IV, HK4, HKIV, HXKP, MODY2



经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

This antibody is not recommended for WB detection of human samples.

Planning an IHC experiment? We recommend our IHCeasy GCK Ready-To-Use IHC Kit. GCK primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19666-1-AP targets GCK in WB, IHC, IF, IP, ELISA applications and shows reactivity with mouse, rat, human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity mouse, rat, human
Cited Reactivitymouse, rat
Immunogen GCK fusion protein Ag8116 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glucokinase (hexokinase 4)
Synonyms GCK, GK, GLK, Glucokinase, glucokinase (hexokinase 4), Hexokinase 4, Hexokinase D, Hexokinase type IV, HHF3, HK IV, HK4, HKIV, HXKP, MODY2
Calculated Molecular Weight 52 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC001890
Gene Symbol GCK
Gene ID (NCBI) 2645
RRIDAB_10863656
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35557
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa.

实验方案

Product Specific Protocols
WB protocol for GCK antibody 19666-1-APDownload protocol
IHC protocol for GCK antibody 19666-1-APDownload protocol
IP protocol for GCK antibody 19666-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Sci Transl Med

Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes

Authors - Hitoshi Watanabe
  • KD Validated
mouseWB,FC

Nat Commun

Citrullination of glucokinase is linked to autoimmune diabetes.

Authors - Mei-Ling Yang
mouseWB,IHC

Phytomedicine

Gentiopicroside ameliorates glucose and lipid metabolism in T2DM via targeting FGFR1

Authors - Zhanchi Xu
mouseWB

PLoS Biol

Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.

Authors - Xuxiao He
mouseWB

iScience

Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions

Authors - Xinghong Guo
IF

J Clin Endocrinol Metab

β-cell dedifferentiation in HOMA-βlow and HOMA-βhigh subjects

Authors - Fuyun Kang