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GLI1 Monoclonal antibody

GLI1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig and More (1)

Applications

WB, IF/ICC, ELISA and More (4)

Conjugate

Unconjugated

CloneNo.

1D2B2

Cat no : 66905-1-Ig

Print datasheet

Synonyms

GLI, GLI family zinc finger 1, GLI1, Glioma associated oncogene, Oncogene GLI, Zinc finger protein GLI1



经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, C6 cells, human testis tissue, BxPC-3 cells, NCI-H1299 cells, U-251 cells, mouse liver tissue, rat liver tissue, MCF-7 cells, MDA-MB-231 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66905-1-Ig targets GLI1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with Human, rat, mouse, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity Human, rat, mouse, pig
Cited Reactivityhuman, mouse, rat
Immunogen GLI1 fusion protein Ag27832 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name GLI family zinc finger 1
Synonyms GLI, GLI family zinc finger 1, GLI1, Glioma associated oncogene, Oncogene GLI, Zinc finger protein GLI1
Calculated Molecular Weight 1106 aa, 118 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC013000
Gene Symbol GLI1
Gene ID (NCBI) 2735
RRIDAB_2882232
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLI1, also known as Glioma-associated oncogene, is a 1106 amino acid protein, which belongs to the GLI C2H2-type zinc-finger protein family. GLI1 is detected in testis and Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. GLI1 is tethered in the cytoplasm by binding to SUFU (PubMed:10806483). GLI1 is activated and translocated to the nucleus promoted by interaction with STK36 (PubMed:10806483). Phosphorylation of GLI1 by ULK3 may promote nuclear localization (PubMed:19878745). GLI1 as a transcriptional activator may regulate the transcription of specific genes during normal development (PubMed:19706761). GLI1 plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract and Mediates SHH signaling (PubMed:19706761). GLI1 plays a role in cell proliferation and differentiation via its role in SHH signaling.

实验方案

Product Specific Protocols
WB protocol for GLI1 antibody 66905-1-IgDownload protocol
IF protocol for GLI1 antibody 66905-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Mol Cancer

Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.

Authors - Yang Gu
humanWB,IHC

Nat Commun

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Authors - Rongxuan Zhu
humanWB

J Exp Clin Cancer Res

ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK

Authors - Li Cui
mouseWB

JCI Insight

Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling.

Authors - Hairong Shi
mouseWB,IF,CoIP

Cancer Immunol Res

Remodeling Chondroitin-6-Sulfate-Mediated Immune Exclusion Enhances Anti-PD-1 Response in Colorectal Cancer with Microsatellite Stability.

Authors - Qijing Wu
mouseWB,IF

Phytother Res

Total flavonoid extract from Dracocephalum moldavica L. improves pulmonary fibrosis by reducing inflammation and inhibiting the hedgehog signaling pathway

Authors - Cheng-Hui He