- Featured Product
- KD/KO Validated
GLI1 Monoclonal antibody
GLI1 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat, mouse, pig and More (1)
Applications
WB, IF/ICC, ELISA and More (4)
Conjugate
Unconjugated
CloneNo.
1D2B2
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HEK-293 cells, C6 cells, human testis tissue, BxPC-3 cells, NCI-H1299 cells, U-251 cells, mouse liver tissue, rat liver tissue, MCF-7 cells, MDA-MB-231 cells, pig brain tissue, rat brain tissue, mouse brain tissue |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66905-1-Ig targets GLI1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with Human, rat, mouse, pig samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP |
Tested Reactivity | Human, rat, mouse, pig |
Cited Reactivity | human, mouse, rat |
Immunogen | GLI1 fusion protein Ag27832 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | GLI family zinc finger 1 |
Synonyms | GLI, GLI family zinc finger 1, GLI1, Glioma associated oncogene, Oncogene GLI, Zinc finger protein GLI1 |
Calculated Molecular Weight | 1106 aa, 118 kDa |
Observed Molecular Weight | 150 kDa |
GenBank Accession Number | BC013000 |
Gene Symbol | GLI1 |
Gene ID (NCBI) | 2735 |
RRID | AB_2882232 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P08151 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for GLI1 antibody 66905-1-Ig | Download protocol |
IF protocol for GLI1 antibody 66905-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cancer Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling. | ||
Nat Commun TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling. | ||
J Exp Clin Cancer Res ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK | ||
JCI Insight Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling. | ||
Cancer Immunol Res Remodeling Chondroitin-6-Sulfate-Mediated Immune Exclusion Enhances Anti-PD-1 Response in Colorectal Cancer with Microsatellite Stability. | ||
Phytother Res Total flavonoid extract from Dracocephalum moldavica L. improves pulmonary fibrosis by reducing inflammation and inhibiting the hedgehog signaling pathway |