GRB10 Polyclonal antibody

GRB10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23591-1-AP

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Synonyms

Grb 10, GRB IR, GRB10, GRB10 adapter protein, GRBIR, IRBP, KIAA0207, MEG1, RSS



经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse brain tissue, mouse liver tissue, rat brain tissue
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23591-1-AP is GRB10 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen GRB10 fusion protein Ag20355 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name growth factor receptor-bound protein 10
Synonyms Grb 10, GRB IR, GRB10, GRB10 adapter protein, GRBIR, IRBP, KIAA0207, MEG1, RSS
Calculated Molecular Weight536aa,61 kDa; 594aa,67 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC024285
Gene Symbol GRB10
Gene ID (NCBI) 2887
RRIDAB_2879301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13322
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRB10 is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including INSR and IGF1R. It may play a role in mediating ubiquitination of INSR, leading to proteasomal degradation.

实验方案

Product Specific Protocols
WB protocol for GRB10 antibody 23591-1-APDownload protocol
IHC protocol for GRB10 antibody 23591-1-APDownload protocol
IF protocol for GRB10 antibody 23591-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Authors - Fei Peng
mouseWB

J Clin Invest

TSC1 regulates the balance between effector and regulatory T cells.

Authors - Park Yoon Y
humanWB

Theranostics

PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex.

Authors - Xiaoxiao Wang
humanWB

Cancer Cell Int

GRB10 is a novel oncogene associated with cell proliferation and prognosis in glioma.

Authors - Yuanbing Chen
humanWB,IHC

Reproduction

MiR199a is implicated in embryo implantation by regulating Grb10 in rat.

Authors - Xia Hong-Fei HF
humanWB

DNA Cell Biol

Modulation of IGF1R Signaling Pathway by GIGYF1 in High Glucose-Induced SHSY-5Y Cells.

Authors - Ting Zhou