验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A431 cells, rat brain tissue, rat spleen tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10254-2-AP targets GRB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | GRB2 fusion protein Ag0395 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | growth factor receptor-bound protein 2 |
| Synonyms | Growth factor receptor-bound protein 2, Grb33, EGFRBP GRB2, ASH |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25-28 kDa |
| GenBank Accession Number | BC000631 |
| Gene Symbol | GRB2 |
| Gene ID (NCBI) | 2885 |
| RRID | AB_2263577 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62993 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-beta protein precursor. Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GRB2 antibody 10254-2-AP | Download protocol |
| IHC protocol for GRB2 antibody 10254-2-AP | Download protocol |
| IF protocol for GRB2 antibody 10254-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway. | ||
Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. | ||
Cancer Lett Deubiquitinase PSMD14 enhances hepatocellular carcinoma growth and metastasis by stabilizing GRB2. | ||
Food Funct Extraction, purification and anticancer activity studies on triterpenes from pomegranate peel | ||
Cancer Lett Melittin inhibits tumor growth and decreases resistance to gemcitabine by downregulating cholesterol pathway gene CLU in pancreatic ductal adenocarcinoma. |
