验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue, human brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
13040-1-AP targets GRID1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | GRID1 fusion protein Ag3694 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | glutamate receptor, ionotropic, delta 1 |
| Synonyms | KIAA1220, Glutamate receptor ionotropic, delta-1, GluR delta-1 subunit, GluR delta 1 subunit, GluD1 |
| Calculated Molecular Weight | 1009 aa, 112 kDa |
| Observed Molecular Weight | 100-112 kDa |
| GenBank Accession Number | BC039263 |
| Gene Symbol | GRID1 |
| Gene ID (NCBI) | 2894 |
| RRID | AB_10638439 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULK0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GRID1 (Glutamate Ionotropic Receptor Delta Type Subunit 1) is a protein-coding gene that belongs to the family of ionotropic glutamate receptors. These receptors are crucial for rapid excitatory synaptic transmission and synaptic plasticity in the central nervous system. GRID1 is primarily expressed in various brain regions, including the cortex, hippocampus, amygdala, striatum, thalamus, nucleus accumbens, lateral hooked nucleus, and dorsal raphe nucleus. In the hippocampus, GRID1 is specifically expressed in the granule cells of the dentate gyrus.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GRID1 antibody 13040-1-AP | Download protocol |
| IHC protocol for GRID1 antibody 13040-1-AP | Download protocol |
| IP protocol for GRID1 antibody 13040-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
