Hemoglobin Alpha-Specific Polyclonal antibody
Hemoglobin Alpha-Specific Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human heart tissue, human spleen tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
19990-1-AP targets Hemoglobin Alpha-Specific in WB, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | hemoglobin, alpha 1 |
Synonyms | Alpha globin, HBA1, HBA1-Specific, HBA2, Hemoglobin alpha chain, Hemoglobin subunit alpha, hemoglobin, alpha 1 |
Calculated Molecular Weight | 16 kDa |
Observed Molecular Weight | 14-16 kDa |
GenBank Accession Number | NM_000558 |
Gene Symbol | HBA1 |
Gene ID (NCBI) | 3039 |
RRID | AB_11182500 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P69905 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). The antibody is specific to HBA1and has no cross reaction to other globin member.
实验方案
Product Specific Protocols | |
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WB protocol for Hemoglobin Alpha-Specific antibody 19990-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |