验证数据展示
经过测试的应用
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, A431 cells |
Planning an IHC experiment? We recommend our IHCeasy HDGFL2 Ready-To-Use IHC Kit. HDGFL2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15134-1-AP targets HDGF2 in IHC, IF/ICC, CoIP, chIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF, CoIP, chIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | HDGF2 fusion protein Ag7281 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hepatoma-derived growth factor-related protein 2 |
| 别名 | HDGFRP2, HRP 2, Hepatoma-derived growth factor 2, HDGF-related protein 2, HDGFL2 |
| 计算分子量 | 74 kDa |
| GenBank蛋白编号 | BC000755 |
| 基因名称 | HDGF2 |
| Gene ID (NCBI) | 84717 |
| RRID | AB_2117220 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z4V5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HDGF2, also known as HDGFL2 (Hepatoma-Derived Growth Factor-like 2), is a gene that encodes a member of the hepatoma-derived growth factor (HDGF) family. HDGF2 plays a role in LEDGF/p75-independent HIV-1 replication by determining HIV-1 integration site selection. It is also involved in the binding to methylated histones, which suggests a role in epigenetic regulation.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for HDGF2 antibody 15134-1-AP | Download protocol |
| IF protocol for HDGF2 antibody 15134-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Transl Med Mis-spliced transcripts generate de novo proteins in TDP-43-related ALS/FTD | ||
Sci Adv LEDGF and HDGF2 relieve the nucleosome-induced barrier to transcription in differentiated cells. | ||
Nucleic Acids Res HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.
| ||
Nucleic Acids Res Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination. | ||
Mov Disord TDP-43 Cryptic RNAs in Perry Syndrome: Differences across Brain Regions and TDP-43 Proteinopathies |
