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HEBP1 Polyclonal antibody

HEBP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16842-1-AP

Print datasheet

Synonyms

HBP, HEBP, HEBP1, heme binding protein 1, p22HBP

验证数据展示

  • WB analysis using 16842-1-AP

    WB analysis using 16842-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16842-1-AP (HEBP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 16842-1-AP

    WB analysis of A549 using 16842-1-AP

    WB result of HEBP1 antibody (16842-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HEBP1 transfected A549 cells.

  • IHC staining of human liver cancer using 16842-1-AP

    IHC staining of human liver cancer using 16842-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16842-1-AP (HEBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inL02 cells, A549 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16842-1-AP targets HEBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HEBP1 fusion protein Ag10538 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heme binding protein 1
Synonyms HBP, HEBP, HEBP1, heme binding protein 1, p22HBP
Calculated Molecular Weight 189 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC016277
Gene Symbol HEBP1
Gene ID (NCBI) 50865
RRIDAB_2117378
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRV9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HEBP1 (heme binding protein 1) is an intracellular tetrapyrrole-binding protein. Catalog#16842-1-AP is a rabbit polyclonal antibody raised against the full-length of human HEBP1. The MW of this protein is 21 kDa, and this antibody specially recognises the 21 kDa protein.

实验方案

Product Specific Protocols
WB protocol for HEBP1 antibody 16842-1-APDownload protocol
IHC protocol for HEBP1 antibody 16842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols