验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse heart tissue, MCF-7 cells, 4T1 cells |
| Positive IP detected in | mouse heart tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10597-1-AP targets HEY2 in WB, IHC, IF, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, CoIP, chIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | HEY2 fusion protein Ag0913 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | hairy/enhancer-of-split related with YRPW motif 2 |
| Synonyms | GRL, GRIDLOCK, Class B basic helix-loop-helix protein 32, CHF1, Cardiovascular helix-loop-helix factor 1 |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 33-40 kDa |
| GenBank Accession Number | BC007707 |
| Gene Symbol | HEY2 |
| Gene ID (NCBI) | 23493 |
| RRID | AB_2118415 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBP5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system. This antibody is a rabbit polyclonal antibody raised against full length of human HEY2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HEY2 antibody 10597-1-AP | Download protocol |
| IF protocol for HEY2 antibody 10597-1-AP | Download protocol |
| IP protocol for HEY2 antibody 10597-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts. | ||
Nat Neurosci Zeb2 recruits HDAC-NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination. | ||
Nat Commun Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis. | ||
Nat Commun Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells. | ||
Nat Commun Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia. |
