验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells, human testis tissue, Jurkat cells, MCF-7 cells, A375 cells, mouse thymus tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19649-1-AP targets Histone H1.2 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, ChIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, camelus bactrianus |
| Immunogen | Histone H1.2 fusion protein Ag7503 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | histone cluster 1, H1c |
| Synonyms | Histone H1, histone cluster 1, H1c, HIST1H1C, H1F2, H1-2 |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 32-33 kDa |
| GenBank Accession Number | BC002649 |
| Gene Symbol | Histone H1.2 |
| Gene ID (NCBI) | 3006 |
| RRID | AB_10694432 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16403 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histone H1 regulates chromatin competency through its high affinity binding to linker DNA as a structural component. It acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. The calculated molecular weight of Histone H1.2 is 21 kDa, but modified Histone H1.2 is about 32 kDa. (PMID: 18258596)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Histone H1.2 antibody 19649-1-AP | Download protocol |
| IHC protocol for Histone H1.2 antibody 19649-1-AP | Download protocol |
| IF protocol for Histone H1.2 antibody 19649-1-AP | Download protocol |
| IP protocol for Histone H1.2 antibody 19649-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells. | ||
Oxid Med Cell Longev The Formation of Melanocyte Apoptotic Bodies in Vitiligo and the Relocation of Vitiligo Autoantigens under Oxidative Stress. | ||
J Neuroinflammation New insights into meningitic Escherichia coli infection of brain microvascular endothelial cells from quantitative proteomics analysis. | ||
Front Immunol HIST1H1C Regulates Interferon-β and Inhibits Influenza Virus Replication by Interacting with IRF3. |
