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HIST1H1T Polyclonal antibody

HIST1H1T Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18188-1-AP

Print datasheet

Synonyms

Histone H1t, Histone H1, Histone, H1t, H1FT

验证数据展示

  • WB analysis using 18188-1-AP

    WB analysis using 18188-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18188-1-AP (HIST1H1T antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 18188-1-AP

    IHC staining of mouse testis using 18188-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18188-1-AP (HIST1H1T antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 18188-1-AP

    IHC staining of mouse testis using 18188-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18188-1-AP (HIST1H1T antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 18188-1-AP

    IF Staining of HepG2 using 18188-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIST1H1T antibody (18188-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inhuman testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18188-1-AP targets HIST1H1T in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen HIST1H1T fusion protein Ag12908 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone cluster 1, H1t
Synonyms Histone H1t, Histone H1, Histone, H1t, H1FT
Calculated Molecular Weight 206 aa, 22 kDa
Observed Molecular Weight22-30 kDa
GenBank Accession NumberBC069517
Gene Symbol HIST1H1T
Gene ID (NCBI) 3010
RRIDAB_2878515
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22492
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Testis-specific histone variants facilitate histone to protamine exchange during maturation of spermatozoa. Three testis-specific histone variants are regulated in male germ cells including H1T, H1T2 and HILS1 (encoded by H1ST1H1T, H1T2 and HILS1 respectively).

实验方案

Product Specific Protocols
WB protocol for HIST1H1T antibody 18188-1-APDownload protocol
IHC protocol for HIST1H1T antibody 18188-1-APDownload protocol
IF protocol for HIST1H1T antibody 18188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Cycle

Function of RAD6B and RNF8 in spermatogenesis.

Authors - Yingli Guo
View Article
mouseIF

Biol Reprod

UHRF1 is indispensable for meiotic sex chromosome inactivation and interacts with the DNA damage response pathway in mice.

Authors - Mengneng Xiong
View Article