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HMBS Polyclonal antibody

HMBS Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14797-1-AP

Print datasheet

Synonyms

Porphobilinogen deaminase, PBG-D, PBGD, PBG D, hydroxymethylbilane synthase

验证数据展示

  • WB analysis of HeLa using 14797-1-AP

    WB analysis of HeLa using 14797-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 14797-1-AP

    IP experiment of HEK-293 using 14797-1-AP

    IP result of anti-HMBS (IP:14797-1-AP, 4ug; Detection:14797-1-AP 1:700) with HEK-293 cells lysate 2400 ug.

  • IF Staining of MCF-7 using 14797-1-AP

    IF Staining of MCF-7 using 14797-1-AP

    Immunofluorescent analysis of MCF-7 cells, using HMBS antibody 14797-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inHeLa cells, A375 cells, HEK-293 cells, Raji cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inMCF-7 cells, Hela cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14797-1-AP targets HMBS in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HMBS fusion protein Ag6509 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hydroxymethylbilane synthase
Synonyms Porphobilinogen deaminase, PBG-D, PBGD, PBG D, hydroxymethylbilane synthase
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-42 kDa
GenBank Accession NumberBC000520
Gene Symbol HMBS
Gene ID (NCBI) 3145
RRIDAB_2117447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08397
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HMBS antibody 14797-1-APDownload protocol
IF protocol for HMBS antibody 14797-1-APDownload protocol
IP protocol for HMBS antibody 14797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols