- Featured Product
- KD/KO Validated
HMGB1 Polyclonal antibody
HMGB1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, ELISA and More (5)
Conjugate
Unconjugated
254
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse kidney tissue, RAW 264.7 cells, Jurkat cells, NIH/3T3 cells, C6 cells |
Positive IHC detected in | mouse brain tissue, human breast cancer tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10829-1-AP targets HMGB1 in WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, ChIP, RIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, zebrafish |
Immunogen | HMGB1 fusion protein Ag1264 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | high-mobility group box 1 |
Synonyms | HMG-1, HMG1, HMG 1, High mobility group protein B1, High mobility group protein 1 |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 25-30 kDa |
GenBank Accession Number | BC003378 |
Gene Symbol | HMGB1 |
Gene ID (NCBI) | 3146 |
RRID | AB_2232989 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P09429 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HMGB1 antibody 10829-1-AP | Download protocol |
IHC protocol for HMGB1 antibody 10829-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
ACS Nano Tumor-Acidity and Bioorthogonal Chemistry-Mediated On-Site Size Transformation Clustered Nanosystem to Overcome Hypoxic Resistance and Enhance Chemoimmunotherapy. | ||
ACS Nano Mitochondria-Targeting Polymer Micelle of Dichloroacetate Induced Pyroptosis to Enhance Osteosarcoma Immunotherapy. | ||
ACS Nano Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy | ||
Nat Commun Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer. | ||
Small An NIR-Fluorophore-Based Theranostic for Selective Initiation of Tumor Pyroptosis-Induced Immunotherapy. | ||
Nat Commun VCP recruitment to mitochondria causes mitophagy impairment and neurodegeneration in models of Huntington's disease. |