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  • KD/KO Validated

HMGCR Polyclonal antibody

HMGCR Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13533-1-AP

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Synonyms

HMG CoA reductase, HMGCR



经过测试的应用

Positive IHC detected inhuman liver cancer tissue, human small intestine tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13533-1-AP targets HMGCR in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsIHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen HMGCR fusion protein Ag4444 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Synonyms HMG CoA reductase, HMGCR
Calculated Molecular Weight 888 aa, 95 kDa
GenBank Accession NumberBC033692
Gene Symbol HMGCR
Gene ID (NCBI) 3156
RRIDAB_2877957
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04035
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HMGCR(3-hydroxy-3-methylglutaryl-coenzyme A reductase) is also named as HMG-CoA reductase and belongs to the HMG-CoA reductase family. This protein exists as a 97-kDa glycoprotein in the endoplasmic reticulum (ER) and is the rate-determining enzyme of the biosynthesis pathway(PMID:15830340).The ectopic expression of either full-length HMGCR or its novel splice variant promotes dysregulation of the MVA pathway. And the ectopic expression of cHMGCR-FL(60 kDa) and cHMGCR-D13(55 kDa) can be detected in HepG2 cells(PMID:20696928).This protein is also can exsit as a oligomer(PMID:20696928).

实验方案

Product Specific Protocols
IHC protocol for HMGCR antibody 13533-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
humanIF

J Cell Biol

TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.

Authors - Wan Yun Ho
  • KD Validated
humanIHC

Acta Pharmacol Sin

Identification of HMGCR as the anticancer target of physapubenolide against melanoma cells by in silico target prediction.

Authors - Hai-Yan Wang
  • KD Validated
human

Cell Mol Gastroenterol Hepatol

A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.

Authors - Dayan Sun
human

PLoS Pathog

Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival.

Authors - Liang Wei Wang
humanIHC

Mol Metab

LDLR inhibition promotes hepatocellular carcinoma proliferation and metastasis by elevating intracellular cholesterol synthesis through the MEK/ERK signaling pathway.

Authors - Ziye Chen