验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human brain tissue, human testis tissue, HepG2 cells, A431 cells, LNCaP cells, mouse testis tissue, mouse colon tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14914-1-AP targets HN1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | HN1 fusion protein Ag6712 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | hematological and neurological expressed 1 |
| Synonyms | Androgen regulated protein 2, ARM2, HN1, HN1A |
| Calculated Molecular Weight | 11 kDa, 16 kDa, 19 kDa |
| Observed Molecular Weight | 23-30 kDa |
| GenBank Accession Number | BC001420 |
| Gene Symbol | HN1 |
| Gene ID (NCBI) | 51155 |
| RRID | AB_10863118 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UK76 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HN1 (hematopoietic- and neurologic-expressed sequence 1) is a highly conserved protein that is expressed in developing and regenerating tissues. Overexpression of HN1 has been reported in various tumors like glioma and breast cancer. Hn1 has also been reported as a marker for human ovarian carcinoma and can distinguish epithelial ovarian carcinoma cells from normal ovarian surface epithelial cells. This collective information suggests that HN1 is involved in processes associated with cell proliferation, repair and/or growth.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HN1 antibody 14914-1-AP | Download protocol |
| IHC protocol for HN1 antibody 14914-1-AP | Download protocol |
| IP protocol for HN1 antibody 14914-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Lett HN1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by interacting with STMN1.
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Kaohsiung J Med Sci Increased expression of hematological and neurological expressed 1 (HN1) is associated with a poor prognosis of hepatocellular carcinoma and its knockdown inhibits cell growth and migration partly by down-regulation of c-Met.
| ||
Chem Biol Drug Des Matrine exerts an anti-tumor effect via regulating HN1 in triple breast cancer both in vitro and in vivo | ||
Cancer Lett Epigenetic inhibition of CTCF by HN1 promotes dedifferentiation and stemness of anaplastic thyroid cancer | ||
Reprod Sci Hematological and Neurological Expressed 1 Promotes Tumor Progression Through mTOR Signaling in Ovarian Cancer
| ||
Biochem Genet Knocking Down HN1 Blocks Helicobacter pylori-Induced Malignant Phenotypes in Gastric Mucosal Cells and Inhibits Gastric Cancer Cell Proliferation, Cytoskeleton Remodeling, and Migration |
