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HSD17B4 Polyclonal antibody

HSD17B4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15116-1-AP

Print datasheet

Synonyms

D-bifunctional protein, D bifunctional protein, 17-beta-hydroxysteroid dehydrogenase 4, 17-beta-HSD 4, 17 beta HSD 4



经过测试的应用

Positive WB detected inmouse liver tissue, mouse brain tissue, mouse heart tissue, HepG2 cells, rat liver tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue, mouse liver tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells
Planning an IHC experiment? We recommend our IHCeasy HSD17B4 Ready-To-Use IHC Kit. HSD17B4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 15 publications below

产品信息

15116-1-AP targets HSD17B4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, sheep
Immunogen HSD17B4 fusion protein Ag7165 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hydroxysteroid (17-beta) dehydrogenase 4
Synonyms D-bifunctional protein, D bifunctional protein, 17-beta-hydroxysteroid dehydrogenase 4, 17-beta-HSD 4, 17 beta HSD 4
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 80 kDa, 45 kDa
GenBank Accession NumberBC003098
Gene Symbol HSD17B4
Gene ID (NCBI) 3295
RRIDAB_2119959
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51659
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSD17B4 (17-beta-hydroxysteroid dehydrogenase 4) is also named as Peroxisomal multifunctional enzyme type 2, D-bifuntional protein or multifunctional protein 2. It codes for a 80 kDa enzyme containing three distinct functional domains and is localized in peroxisomes. It is a bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids and catalyzing the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-brancked-chian fatty acids. After peroxisomal import, the full-length protein is proteolytically cleaved to yield a 35-kDa dehydrogenase subunit and a 45-kDa hydratase subunit containing the hydratase and SCP domains (PMID: 28868548, 24602372).

实验方案

Product Specific Protocols
WB protocol for HSD17B4 antibody 15116-1-APDownload protocol
IHC protocol for HSD17B4 antibody 15116-1-APDownload protocol
IF protocol for HSD17B4 antibody 15116-1-APDownload protocol
IP protocol for HSD17B4 antibody 15116-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Commun Biol

Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism

Authors - Michael Landowski
mouseWB

Neurobiol Dis

Peroxisomal multifunctional protein-2 deficiency causes neuroinflammation and degeneration of Purkinje cells independent of very long chain fatty acid accumulation.

Authors - Verheijden Simon S
WB

Sci Rep

Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells.

Authors - Mitsuki Nozaki
mouseWB

Cells

Cell Type-Selective Loss of Peroxisomal β-Oxidation Impairs Bipolar Cell but Not Photoreceptor Survival in the Retina.

Authors - Daniëlle Swinkels
humanWB

Mol Cells

The Peroxisomal Localization of Hsd17b4 Is Regulated by Its Interaction with Phosphatidylserine.

Authors - Sang-Ah Lee
humanWB

Biology (Basel)

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Authors - Olga Gourdomichali